To remove stubborn creases in fabric caused by the fabric being folded and packaged, sometimes ironing alone isn't enough. Gently hand wash the finished stitching, then place on a folded thick towel and then with a cool iron, iron until dry. Ironing on the back of the fabric will make the stitches raised, and you might be surprised at the results.
Avoid using detergents with lemon scent or fragrance as these will discolour some threads (especially metallic ones).
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